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#MIDNIGHT PROTOCOL NANOPORE FULL#
Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday with Australia and New Zealand leaving our warehouses on a Friday. Products are shipped to customers within the USA and EU Monday to Thursday. Shipped at 2–8☌ Long-term storage -20☌ Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customerįlow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box. PCR tiling of SARS-CoV-2 virus with rapid barcoding and Midnight RT PCR Expansion (SQK-RBK110.96 and EXP-MRT001) The Midnight RT PCR Expansion features: Feature As new mutations are identified in the SARS-CoV-2 lineages, we will continue to work closely with the community (including the entire ARTIC Nextwork, Nikki Freed, Olin Silander, Josh Quick, John Tyson, Nick Loman and many more) to rapidly respond and ensure performance of Oxford Nanopore Technologies' protocols are always kept up-to-date, providing the best performance for robust SARS-CoV-2 whole genome sequencing.ĭeconvolution of barcoded sequencing data is supported by MinKNOW and Guppy software, which classify the barcode sequencing and sort reads into corresponding folders.įlow Cell Wash Kits can be purchased here for running fewer numbers of barcodes. From the 1st February, we have included an additional primer 28 to address the drop out observed with the Omicron variant. The primers provided in this kit were initially designed by Freed et al., 2020, using the Primal Scheme. This expansion enables PCR tiling to generate overlapping amplicons across the whole SARS-CoV-2 genome before using the Rapid Barcoding Kit 96 (SQK-RBK110.96) to barcode and pool multiple samples together for sequencing. The expansion kit provides all reagents and primers required to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples, without the requirement of purchasing further components from multiple external suppliers. The Midnight RT PCR Expansion (EXP-MRT001) is designed to be used with the Rapid Barcoding Kit 96 (SQK-RBK110.96) to provide all reagents needed for the complete workflow to sequence SARS-CoV-2 RNA samples. Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail. Wish to multiplex samples to reduce price per sample.
Run in sets of 24* - 96 samples per flow cell The Midnight bundle includes everything you need to sequence SARS-CoV-2: Feature Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow. The protocol delivers robust full genome coverage across a wide range of Ct values. It allows rapid turn-around-times that can assist with monitoring of novel variants of concern.
This method is simple and quick to deploy. By amplifying longer amplicons, Oxford Nanopores rapid chemistry can be deployed enabling users to rapidly attach a barcode, pool samples and adapt them for sequencing. The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long).
Midnight Expansion Kit with RT-PCR reagents and primers to amplify SARS-CoV-2 from input RNA.A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples:
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